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1H-NMR study of the structural influence of Y64 substitution in Desulfovibrio vulgaris Hildenborough cytochrome c553.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1998 Feb 01; Vol. 251 (3), pp. 787-94. - Publication Year :
- 1998
-
Abstract
- Y64 has been replaced in cytochrome c553 from Desulfovibrio vulgaris Hildenborough by phenylalanine, leucine, valine, serine and alanine residues. An NMR study of structural variation induced in both oxidoreduction states of the molecule has been carried out by analysing observed chemical-shift variations. Dynamic changes were evidenced using NH exchange. We have observed that the substitution has a drastic effect on the stability of the molecule in the reduced state, although there is no effect on the reduction potential of the cytochrome. Y64-->F substitution induces particular effects on the NH exchange at the N-terminal, C-terminal and central alpha-helices and increases the stability of the oxidized molecule.
- Subjects :
- Amino Acid Sequence
Amino Acid Substitution
Conserved Sequence
Hydrogen
Mutagenesis, Site-Directed
Nuclear Magnetic Resonance, Biomolecular methods
Oxidation-Reduction
Protein Folding
Recombinant Proteins chemistry
Cytochrome c Group chemistry
Desulfovibrio vulgaris metabolism
Protein Conformation
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 251
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9490053
- Full Text :
- https://doi.org/10.1046/j.1432-1327.1998.2510787.x