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Ectopic expression of DAN enhances the retinoic acid-induced neuronal differentiation in human neuroblastoma cell lines.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1998 Feb 24; Vol. 243 (3), pp. 722-6. - Publication Year :
- 1998
-
Abstract
- Retinoic acid (RA) plays a major role in neuronal cell differentiation. Neuroblastoma cells differentiate in vitro by extending neurites and forming ganglion-like aggregates in response to RA. In the present study, we have examined a biological role(s) of DAN in the regulation of RA-mediated cellular differentiation in neuroblastoma cells. RTBM1 and SH-SY5Y cells undergo marked morphological changes associated with a remarkable induction of DAN gene expression when exposed to RA. By transfecting an expression vector harboring a rat DAN cDNA into SH-SY5Y cells, we have obtained two independent transfectants which express a large amount of DAN. The forced expression of DAN gene enhanced the neurite extension in the presence of RA, suggesting that DAN gene product might contain some regulatory role(s) in the RA-induced cellular differentiation in neuroblastoma cells.
- Subjects :
- Animals
Blotting, Northern
Cell Cycle Proteins
Cytomegalovirus genetics
Genes, Tumor Suppressor
Humans
Nerve Tissue Proteins
Neurites pathology
Neuroblastoma metabolism
Promoter Regions, Genetic
RNA, Messenger metabolism
Rats
Transfection
Tumor Cells, Cultured
Cell Differentiation drug effects
Gene Expression
Neuroblastoma pathology
Neurons pathology
Proteins genetics
Tretinoin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 243
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9500977
- Full Text :
- https://doi.org/10.1006/bbrc.1998.8112