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The regulatory role of heat shock protein 70-reactive CD4+ T cells during rat listeriosis.
- Source :
-
International immunology [Int Immunol] 1998 Feb; Vol. 10 (2), pp. 117-30. - Publication Year :
- 1998
-
Abstract
- Protection against infection with Listeria monocytogenes depends primarily on Listeria-specific T cells. We show here that CD4+ TCR alphabeta+ T cells are capable of recognizing the mycobacterial heat shock protein (HSP) 70, that appears in the peritoneal cavity of F344 rats infected i.p. with L. monocytogenes. The HSP70-reactive CD4+ T cells recognized a peptide comprising 234-252 residues as present in the 70 kDa HSP of Mycobacterium tuberculosis in the context of RT1.B MHC class II molecules. Analysis of TCR Vbeta gene expression with RT-PCR revealed that the HSP70-reactive CD4+ T cells predominantly used the Vbeta16 gene segment, whereas the heat-killed Listeria (HKL)-specific T cells expressed a diverse set of Vbeta gene segments. In contrast to the HKL-specific T cells producing IFN-gamma, the HSP70-reactive CD4+ T cells produced TGF-beta1 and IL-10 but neither Th1- or Th2-type cytokines. Adoptive transfer with HSP70-reactive T cells rendered rats susceptible to listerial infection. Collectively, these results proposed that the HSP70-reactive CD4+ T cells appearing during rat listeriosis may be involved in termination of Th1 cell-mediated excessive inflammation after the battle against L. monocytogenes has been won.
- Subjects :
- Adoptive Transfer
Amino Acid Sequence
Animals
Antigen-Presenting Cells immunology
Antigens, Bacterial immunology
Epitopes
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
Histocompatibility Antigens Class II immunology
Immunophenotyping
Interleukin-10 biosynthesis
Lymphocyte Activation
Molecular Sequence Data
Peritoneal Cavity cytology
Rats
Rats, Inbred F344
Receptors, Antigen, T-Cell, alpha-beta
Transforming Growth Factor beta biosynthesis
Bacterial Proteins immunology
CD4-Positive T-Lymphocytes immunology
HSP70 Heat-Shock Proteins physiology
Listeria monocytogenes immunology
Listeriosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0953-8178
- Volume :
- 10
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9533439
- Full Text :
- https://doi.org/10.1093/intimm/10.2.117