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Serum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system.
- Source :
-
Clinical chemistry [Clin Chem] 1998 Apr; Vol. 44 (4), pp. 749-59. - Publication Year :
- 1998
-
Abstract
- We compared the automated Paragon 2000 clinical capillary zone electrophoresis (CZE) system with two manual methods, agarose electrophoresis (AGE) and cellulose acetate electrophoresis (CAE). Reference intervals in healthy adults were determined for each method. When compared with AGE and CAE, CZE gave substantially higher reference values for the alpha1-globulin fraction. With CZE, within-run precision for fraction quantitation was between 0.5% (albumin) and 4.1% (alpha1-globulin). Total precision was between 0.8% (albumin) and 5.3% (beta-globulin). Data obtained from CZE showed poor linear correlation with results obtained by AGE but good linear correlation with data from CAE. Analysis of serum from patients with inter alia inflammation, nephrotic syndrome, or polyclonal gammopathy showed that clinical information obtained by CZE is comparable with information obtained by AGE and CAE. We conclude that CZE offers a clinically reliable alternative to AGE and CAE and has the advantages of automation, higher precision, and faster turnaround time.
- Subjects :
- Acute-Phase Reaction blood
Adolescent
Adult
Aged
Bilirubin analysis
Blood Protein Electrophoresis
Diabetes Mellitus blood
Electrophoresis, Agar Gel
Electrophoresis, Capillary instrumentation
Electrophoresis, Cellulose Acetate
Female
Hemolysis
Humans
Lipids analysis
Liver Diseases blood
Male
Middle Aged
Nephrotic Syndrome blood
Paraproteinemias blood
Pregnancy
Reference Values
Time Factors
Blood Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9147
- Volume :
- 44
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9554485