[Quantitative analyses of DNA content and P53 gene product expression from epithelial lacrimal gland tumors].

Objective: To investigate the significance of quantitative analyses of DNA content and P53 gene product expression which are applied for pathological diagnosis of epithelial lacrimal gland tumor.
Methods: Flow cytometry (FCM) and immunofluorescence staining technique were used to quantitatively measure the DNA content and expression of P53 gene product from 39 cases with epithelial lacrimal gland tumors.
Results: It is indicated that the pleomorphic adenomas still possess of the properties of DNA diploid of normal lacrimal gland cell, but malignant tumors are characterized by DNA aneuploidy. There is a significant difference in the content of expression of P53 gene product between pleomorphic adenomas and malignant tumors (P < 0.001). The content of expression of P53 gene product is positively correlated to the DNA content, and both are increasing with the reduction of tumor tissue differentiation.
Conclusion: Quantitative analyses of DNA content and expression of P53 gene product may provide objective indexes for the pathological diagnosis of epithelial lacrimal gland tumor.