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Expression of the bglH gene of Lactobacillus plantarum is controlled by carbon catabolite repression.
- Source :
-
Journal of bacteriology [J Bacteriol] 1998 Jul; Vol. 180 (13), pp. 3400-4. - Publication Year :
- 1998
-
Abstract
- A newly identified bglH gene coding for a phospho-beta-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the beta-glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from -3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression of bglH expression.
- Subjects :
- Amino Acid Sequence
Base Sequence
Benzyl Alcohols metabolism
Cloning, Molecular
Consensus Sequence
Genes, Bacterial
Glucose metabolism
Glucosidases chemistry
Glucosidases genetics
Glucosides
Lactobacillus growth & development
Lactobacillus metabolism
Molecular Sequence Data
Open Reading Frames
RNA, Messenger biosynthesis
Recombinant Proteins biosynthesis
Recombinant Proteins chemistry
Sequence Alignment
Sequence Homology, Amino Acid
Transcription, Genetic
Enzyme Repression
Glucosidases biosynthesis
Lactobacillus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 180
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 9642194
- Full Text :
- https://doi.org/10.1128/JB.180.13.3400-3404.1998