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Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1998 Jun 29; Vol. 247 (3), pp. 681-6. - Publication Year :
- 1998
-
Abstract
- Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis.
- Subjects :
- Amino Acid Sequence
Animals
COS Cells
Cloning, Molecular
Evolution, Molecular
Mice
Microsatellite Repeats genetics
Molecular Sequence Data
RNA, Messenger metabolism
Recombinant Proteins chemistry
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Substrate Specificity
Transfection genetics
Arylsulfotransferase chemistry
Sulfotransferases chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 247
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9647753
- Full Text :
- https://doi.org/10.1006/bbrc.1998.8872