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Allelic imbalance of insulin-like growth factor II gene expression in cancerous and precancerous lesions of the liver.
- Source :
-
Hepatology (Baltimore, Md.) [Hepatology] 1998 Jul; Vol. 28 (1), pp. 86-9. - Publication Year :
- 1998
-
Abstract
- Allelic imbalance of the insulin-like growth factor II (IGF II) gene expression is often seen in hepatocellular carcinoma (HCC). To investigate the role of allelic imbalance in hepatocarcinogenesis, we have studied allelic expression status of the IGF II gene in dysplastic nodules, which are precancerous lesions of HCC, as well as in HCCs of different histological grade, and the influence of the allelic imbalance on IGF II gene expression has also been examined. Allelic imbalance was observed in 3 of 7 dysplastic nodules, in 7 of 9 well-differentiated HCCs, and in 8 of 9 moderately differentiated HCCs. IGF II gene expression level, which was studied by a semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR), was significantly higher (3.6-fold) in the dysplastic nodules than the control livers, but a significant increase in the IGF II gene expression was not observed in well- and moderately differentiated HCCs as compared with the control livers. These results demonstrate that the allelic imbalance of the IGF II gene expression is seen in the early stage (precancerous lesions) of hepatocarcinogenesis. Association of the allelic imbalance with an increased expression of the IGF II gene in the precancerous lesions might suggest a possible involvement of an IGF II autocrine loop in the pathogenesis of these lesions.
- Subjects :
- Carcinoma, Hepatocellular pathology
Humans
Liver physiology
Liver Neoplasms pathology
Loss of Heterozygosity
Polymerase Chain Reaction
Reference Values
Transcription, Genetic
Alleles
Carcinoma, Hepatocellular genetics
Gene Expression physiology
Insulin-Like Growth Factor II genetics
Liver Neoplasms genetics
Precancerous Conditions genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0270-9139
- Volume :
- 28
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Hepatology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 9657100
- Full Text :
- https://doi.org/10.1002/hep.510280113