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pH-regulated electroretention chromatography: towards a new method for the separation of proteins according to their isoelectric points.
- Source :
-
Electrophoresis [Electrophoresis] 1998 Jun; Vol. 19 (7), pp. 1135-9. - Publication Year :
- 1998
-
Abstract
- The pH-dependent electroretention behavior of model proteins cytochrome c and ribonuclease A was studied in a hollow fiber arrangement, similar to that used in electrical field-flow fractionation. Field-induced immobilization of the proteins at the inner wall of the fiber was a function of the pH adjusted in the solution surrounding it, indicating that the pH inside the fiber lumen, relevant for protein migration, quickly equilibrates to the regulated value outside. A complete separation of the model proteins was achieved. Advantages of the principle as well as prospects for the development of a technique separating more than two protein species according to their isoelectric points are discussed.
- Subjects :
- Animals
Cattle
Chemical Fractionation methods
Chromatography instrumentation
Cytochrome c Group isolation & purification
Electrophoresis, Capillary methods
Horses
Hydrogen-Ion Concentration
Isoelectric Focusing methods
Isoelectric Point
Ribonuclease, Pancreatic isolation & purification
Chromatography methods
Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0173-0835
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Electrophoresis
- Publication Type :
- Academic Journal
- Accession number :
- 9662175
- Full Text :
- https://doi.org/10.1002/elps.1150190713