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A negative arterial-portal venous glucose gradient decreases skeletal muscle glucose uptake.
- Source :
-
The American journal of physiology [Am J Physiol] 1998 Jul; Vol. 275 (1), pp. E101-11. - Publication Year :
- 1998
-
Abstract
- The effect of a negative arterial-portal venous (a-pv) glucose gradient on skeletal muscle and whole body nonhepatic glucose uptake was studied in 12 42-h-fasted conscious dogs. Each study consisted of a 110-min equilibration period, a 30-min baseline period, and two 120-min hyperglycemic (2-fold basal) periods (either peripheral or intraportal glucose infusion). Somatostatin was infused along with insulin (3 x basal) and glucagon (basal). Catheters were inserted 17 days before studies in the external iliac artery and hepatic, portal and common iliac veins. Blood flow was measured in liver and hindlimb using Doppler flow probes. The arterial blood glucose, arterial plasma insulin, arterial plasma glucagon, and hindlimb glucose loads were similar during peripheral and intraportal glucose infusions. The a-pv glucose gradient (in mg/dl) was 5 +/- 1 during peripheral and -18 +/- 3 during intraportal glucose infusion. The net hindlimb glucose uptakes (in mg/min) were 5.0 +/- 1.2, 20.4 +/- 4.5, and 14.8 +/- 3.2 during baseline, peripheral, and intraportal glucose infusion periods, respectively (P < 0.01, peripheral vs. intraportal); the hindlimb glucose fractional extractions (in %) were 2.8 +/- 0.4, 4.7 +/- 0.8, and 3.9 +/- 0.5 during baseline, peripheral, and intraportal glucose infusions, respectively (P < 0. 05, peripheral vs. intraportal). The net whole body nonhepatic glucose uptakes (in mg . kg-1 . min-1) were 1.6 +/- 0.1, 7.9 +/- 1.3, and 5.4 +/- 1.1 during baseline, peripheral, and intraportal glucose infusion, respectively (P < 0.05, peripheral vs. intraportal). In the liver, net glucose uptake was 70% greater during intraportal than during peripheral glucose infusion (5.8 +/- 0.7 vs. 3.4 +/- 0.4 mg . kg-1 . min-1). In conclusion, despite comparable glucose loads and insulin levels, hindlimb and whole body net nonhepatic glucose uptake decreased significantly during portal venous glucose infusion, suggesting that a negative a-pv glucose gradient leads to an inhibitory signal in nonhepatic tissues, among which skeletal muscle appears to be the most important.
- Subjects :
- Animals
Blood Glucose drug effects
Dogs
Female
Glucagon administration & dosage
Glucagon blood
Glucagon pharmacology
Hepatic Artery physiology
Hindlimb
Hyperglycemia metabolism
Iliac Artery physiology
Iliac Vein physiology
Infusions, Intravenous
Insulin administration & dosage
Insulin blood
Insulin pharmacology
Liver metabolism
Liver Circulation physiology
Male
Muscle, Skeletal blood supply
Muscle, Skeletal drug effects
Portal System physiology
Regional Blood Flow
Somatostatin administration & dosage
Somatostatin blood
Somatostatin pharmacology
Blood Glucose metabolism
Glucose metabolism
Muscle, Skeletal physiology
Portal Vein physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 275
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 9688880
- Full Text :
- https://doi.org/10.1152/ajpendo.1998.275.1.E101