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HrWnt-5: a maternally expressed ascidian Wnt gene with posterior localization in early embryos.
- Source :
-
The International journal of developmental biology [Int J Dev Biol] 1998 May; Vol. 42 (4), pp. 573-9. - Publication Year :
- 1998
-
Abstract
- Ascidians show a highly determinate mode of development. In particular, components of the posterior-vegetal cytoplasm of fertilized eggs are responsible for the establishment of the embryonic axis. Recent studies have, however, also revealed significant roles of cell-cell interactions during embryogenesis. Proteins encoded by the Wnt family of genes act as signals and have been shown to play important roles in a wide range of developmental processes. Here we have isolated and characterized an ascidian Wnt gene, HrWnt-5, from Halocynthia roretzi. HrWnt-5 mRNA is present in the vegetal cortex in unfertilized eggs. After fertilization, HrWnt-5 mRNA moves to the equatorial region to form a crescent-like structure, after which the mRNA is concentrated in the posteriormost region of the embryo. This early pattern of HrWnt-5 mRNA localization coincides with another posterior-vegetally localized mRNA, pem, isolated from Ciona savignyi. In the gastrula, the zygotic HrWnt-5 mRNA is found in a variety of blastomeres, suggesting multiple roles of the gene.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blastomeres chemistry
Cell Polarity
Cloning, Molecular
DNA, Complementary genetics
Gastrula chemistry
Gene Dosage
Genes genetics
Molecular Sequence Data
Phylogeny
RNA, Messenger analysis
Wnt Proteins
Egg Proteins genetics
Gene Expression Regulation, Developmental physiology
Urochordata embryology
Urochordata genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0214-6282
- Volume :
- 42
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The International journal of developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 9694628