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Predictive model for capillary electrophoretic peptide mobility in 2,2,2-trifluoroethanol-water solution.
- Source :
-
Electrophoresis [Electrophoresis] 1998 Jul; Vol. 19 (10), pp. 1728-32. - Publication Year :
- 1998
-
Abstract
- Using capillary electrophoresis (CE) on a set of 21 peptides with a molecular mass ranging from about 350 to 1850 Da, the Stokes radii at different protonation stages and the acidic dissociation constants in water and in a 2,2,2-trifluoroethanol (TFE) water mixture (30% v/v) were determined. These results permitted us to establish separately the reliability of semiempirical models utilized for the prediction of peptide size and charge at different acidic pHapp (pHapp range: 2.00-4.25). The data obtained on size and charge were utilized in order to provide suitable mobility predictions on the basis of the charge-to-size ratio. The best predictive conditions for size and charge were found at the most acidic range of pHapp studied (2.00-2.25), either in water or a TFE-water mixture, and reliable predictive equations for peptide mobility were established at this pHapp.
Details
- Language :
- English
- ISSN :
- 0173-0835
- Volume :
- 19
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Electrophoresis
- Publication Type :
- Academic Journal
- Accession number :
- 9719552
- Full Text :
- https://doi.org/10.1002/elps.1150191033