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Protein I/II of oral viridans streptococci increases expression of adhesion molecules on endothelial cells and promotes transendothelial migration of neutrophils in vitro.
- Source :
-
Cellular immunology [Cell Immunol] 1998 Aug 01; Vol. 187 (2), pp. 145-50. - Publication Year :
- 1998
-
Abstract
- As accumulation of leukocytes in perivascular tissues is a key step in inflammatory disorders, we have analyzed in the present work the up-regulation of expression of adhesion molecules, such as E-selectin, ICAM-1, and VCAM-1, on human endothelial cells, in response to protein I/II, a modulin from Streptococcus mutans OMZ 175. Using cultured human saphenous vein endothelial cells (HSVEC), we demonstrated that protein I/II directly and specifically up-regulated E-selectin, ICAM-1, and VCAM-1 expression. We confirmed also that the up-regulation of adhesion molecules in HSVEC is mediated by lectin activity for NANA- and fucose-containing receptors. The ability of protein I/II to promote the transendothelial migration of neutrophils was then examined. Using Transwell inserts, we found that protein I/II, in promoting the up-regulation of adhesion molecule expression, stimulates neutrophil migration through endothelial cells. These events may play a role in the etiology of inflammatory responses leading to the various pathologies associated with oral viridans streptococci.
- Subjects :
- Animals
Cell Movement drug effects
Endothelium, Vascular cytology
Humans
Mice
Mouth microbiology
Neutrophils physiology
Bacterial Proteins pharmacology
E-Selectin biosynthesis
Endothelium, Vascular metabolism
Intercellular Adhesion Molecule-1 biosynthesis
Membrane Glycoproteins
Neutrophils drug effects
Streptococcus mutans physiology
Vascular Cell Adhesion Molecule-1 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 187
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 9732703
- Full Text :
- https://doi.org/10.1006/cimm.1998.1327