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IL-1beta, IL-6, TNFalpha, fetal fibronectin, and endotoxin in the lower genital tract of pregnant women with bacterial vaginosis.
- Source :
-
Acta obstetricia et gynecologica Scandinavica [Acta Obstet Gynecol Scand] 1998 Aug; Vol. 77 (7), pp. 701-6. - Publication Year :
- 1998
-
Abstract
- Background: In our studies on women with bacterial vaginosis (BV) in early pregnancy a strong association has been found between BV and the levels of endotoxin or interleukin-1alpha (IL-1alpha) in the lower genital tract. In the present study we investigated if an association could be found between BV and other cytokines (IL-1beta, IL-6, tumor necrosis factor alpha, TNF) or fetal fibronectin (FFN). The cytokine-inducing capacity of endotoxins present in the cervical mucus was explored in a monocytic cell assay.<br />Methods: Cervical mucus or cervicovaginal fluid was collected from women with (BV) and without BV (nonBV) attending a family planning unit for first trimester abortion. The concentrations of IL-1beta, IL-6, TNF and FFN were determined by quantitative enzyme immunoassays. TNF was determined in 63 women (BV, n=25) out of whom 37 (BV, n=11) were analyzed for IL-1beta and the remaining 26 for IL-6 (BV, n=14). FFN was determined in another 36 women (BV, n= 19). The cytokine-inducing capacity of endotoxin-containing cervical mucus and purified endotoxin of Prevotella bivia were studied by an in vitro cell assay using a human monocytic cell line (THP-1).<br />Results: IL-lbeta and IL-6 were found in almost all women. The levels of IL-1beta, but not IL-6, TNF or FFN, were significantly increased in women with BV compared with the nonBV women (p<0.05). Purified endotoxin from P. bivia, and cervical mucus from BV women containing high levels of endotoxin were able to induce a cytokine response (IL-6) in monocytic cells in vitro.<br />Conclusion: BV is associated with increased levels of IL-1beta in the lower genital tract of pregnant women in the first trimester. The ability of BV-associated endotoxins to induce cytokine production in monocytic cells may partly explain the increased IL-1beta levels.
- Subjects :
- Adult
Bacteroidaceae Infections immunology
Bacteroidaceae Infections microbiology
Cervix Mucus metabolism
Female
Genitalia, Female immunology
Genitalia, Female microbiology
Humans
Pregnancy
Pregnancy Complications, Infectious immunology
Pregnancy Complications, Infectious microbiology
Pregnancy Trimester, First
Vaginosis, Bacterial immunology
Vaginosis, Bacterial microbiology
Bacteroidaceae Infections metabolism
Endotoxins metabolism
Fibronectins metabolism
Genitalia, Female metabolism
Interleukin-1 metabolism
Interleukin-6 metabolism
Pregnancy Complications, Infectious metabolism
Prevotella
Tumor Necrosis Factor-alpha metabolism
Vaginosis, Bacterial metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0001-6349
- Volume :
- 77
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Acta obstetricia et gynecologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 9740515