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Rat liver mitochondria can hydrolyse thiamine pyrophosphate to thiamine monophosphate which can cross the mitochondrial membrane in a carrier-mediated process.
- Source :
-
FEBS letters [FEBS Lett] 1998 Sep 11; Vol. 435 (1), pp. 6-10. - Publication Year :
- 1998
-
Abstract
- We show here that TPP --> TMP conversion can take place in rat liver mitochondria. This occurs via the novel, putative TPP pyrophosphatase localised in the mitochondrial matrix, as shown both by digitonin titration and by an HPLC enzyme assay carried out on the mitochondrial matrix fraction. Certain features of the reaction, including the substrate and pH dependence, are reported. Additional evidence is given that externally added TMP can cross the mitochondrial membrane in a manner consistent with the occurrence of a carrier-mediated process. This can occur both via the TPP translocator and via a novel translocator, inhibited by CAT but different from the ADP/ATP carrier.
- Subjects :
- Animals
Atractyloside analogs & derivatives
Atractyloside metabolism
Binding, Competitive
Biological Transport
Catalysis
Hydrolysis
Intracellular Membranes enzymology
Male
Mitochondria, Liver enzymology
Rats
Rats, Wistar
Spectrometry, Fluorescence
Thiamine Pyrophosphate antagonists & inhibitors
Intracellular Membranes metabolism
Mitochondria, Liver metabolism
Thiamine Monophosphate metabolism
Thiamine Pyrophosphate metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 435
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 9755848
- Full Text :
- https://doi.org/10.1016/s0014-5793(98)01007-2