Back to Search
Start Over
EBER oligonucleotide RNA in situ hybridization in EBV associated neoplasms.
EBER oligonucleotide RNA in situ hybridization in EBV associated neoplasms.
- Source :
-
Pathology oncology research : POR [Pathol Oncol Res] 1998; Vol. 4 (3), pp. 201-5. - Publication Year :
- 1998
-
Abstract
- In virus associated diseases identification of viruses in cells can contribute to the understanding of the pathogenesis and may also help to establish the diagnosis. In the present communication, the effects of the microwave pretreatment (MWP) and that of the proteinase-K enzymatic predigestion (PKD) on EBER RNA oligonucleotide in situ hybridization (EBER-RNA-ISH) (EBER: Epstein-Barr-Encoded-(Early)-RNA) were studied. The efficacy of two EBV detecting methods, latent membrane protein-1 (LMP-1) immunohistochemistry and EBER-RNA-ISH were also compared. Our results show that microwave pretreatment enhances the intensity of the ISH signals and preserves significantly better the structure of the tissues compared with enzymatic predigestion. EBER-RNA-ISH, mainly in the nasopharyngeal carcinoma cases, showed a more frequent positivity than the immunohistochemical reaction for LMP-1, however in case of the Warthin's tumor only the LMP-1 protein was expressed.
- Subjects :
- Adenolymphoma chemistry
Adenolymphoma pathology
Biomarkers
Burkitt Lymphoma chemistry
Burkitt Lymphoma pathology
Burkitt Lymphoma virology
Carcinoma chemistry
Carcinoma pathology
Epstein-Barr Virus Infections metabolism
Epstein-Barr Virus Infections pathology
Fluorescent Antibody Technique, Indirect
Formaldehyde
Herpesvirus 4, Human genetics
Hodgkin Disease metabolism
Hodgkin Disease pathology
Hodgkin Disease virology
Humans
Lymphoma chemistry
Lymphoma pathology
Lymphoma, Non-Hodgkin chemistry
Lymphoma, Non-Hodgkin pathology
Lymphoma, Non-Hodgkin virology
Lymphoma, T-Cell chemistry
Lymphoma, T-Cell pathology
Lymphoma, T-Cell virology
Microwaves
Nasopharyngeal Neoplasms chemistry
Nasopharyngeal Neoplasms pathology
Salivary Gland Neoplasms chemistry
Salivary Gland Neoplasms pathology
Sensitivity and Specificity
Stomach Neoplasms chemistry
Stomach Neoplasms pathology
Stomach Neoplasms virology
Tissue Fixation
Viral Matrix Proteins analysis
Adenolymphoma virology
Carcinoma virology
Epstein-Barr Virus Infections virology
Herpesvirus 4, Human isolation & purification
In Situ Hybridization methods
Lymphoma virology
Nasopharyngeal Neoplasms virology
RNA, Neoplasm analysis
RNA, Viral analysis
Salivary Gland Neoplasms virology
Subjects
Details
- Language :
- English
- ISSN :
- 1219-4956
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pathology oncology research : POR
- Publication Type :
- Academic Journal
- Accession number :
- 9761938
- Full Text :
- https://doi.org/10.1007/BF02905249