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Temporal expression of TGF-beta1, EGF, and PDGF-BB in a model of colonic wound healing.
- Source :
-
The Journal of surgical research [J Surg Res] 1998 Nov; Vol. 80 (1), pp. 52-7. - Publication Year :
- 1998
-
Abstract
- Background: Dehiscence of colonic anastomoses is a multifactorial phenomenon. One mechanism by which this can occur is a deficiency of colonic submucosal collagen. Peptide growth factors (PGFs) have been shown to play a role in the synthesis, deposition, and maturation of collagen. Specifically, in tissues other than the colon, the transforming growth factor-beta (TGF-beta1) gene has been shown to be temporally associated with expression of the procollagen gene. This study examines the temporal expression of the TGF-beta1, epidermal growth factor (EGF), and platelet-derived growth factor B (PDGF-BB) genes and their temporal relationship to the expression of the procollagen type 1 (PROC I) gene.<br />Materials and Methods: Forty-eight Sprague-Dawley rats underwent transection of the descending colon with primary anastomosis. Perianastomotic colonic tissue was harvested on Day 0 and postoperative days 3, 5, 6, 7, and 14. Colonic tissue was analyzed using semiquantitative reverse transcriptase-polymerase chain reaction and primers specific for the TGF-beta1, EGF, and PDGF-B growth factors. Relative expression ratios of PGFs and PROC I genes were calculated versus a constitutive gene.<br />Results: The data show that although all three of the PGFs genes were expressed in healing postoperative colonic tissue, only TGF-beta1 showed a significant increase in its level of expression versus a constitutive gene from a mean ratio of 0.4 +/- 0. 08 on Day 0 to a mean ratio of 1.9 +/- 0.27 on Day 7 (P < 0.0001 by ANOVA). The PROC I gene also showed a significant increase in expression (P < 0.001 by ANOVA) in the postoperative period which temporally correlated with the increase in the expression of the TGF-beta1 gene (r = 0.89, P < 0.05).<br />Conclusions: The temporal correlation between an increase in the gene expression of TGF-beta1 and PROC I is initial evidence that that TGF-beta1 plays a significant role in collagen metabolism in a healing colonic anastomosis.<br /> (Copyright 1998 Academic Press.)
- Subjects :
- Animals
Becaplermin
Male
Procollagen genetics
Proto-Oncogene Proteins c-sis
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Colon injuries
Epidermal Growth Factor genetics
Gene Expression Regulation physiology
Platelet-Derived Growth Factor genetics
Transforming Growth Factor beta genetics
Wound Healing physiology
Wounds, Penetrating physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-4804
- Volume :
- 80
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 9790814
- Full Text :
- https://doi.org/10.1006/jsre.1998.5326