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Activation of Na+, H+ exchanger produces vasoconstriction of renal resistance vessels.
- Source :
-
American journal of hypertension [Am J Hypertens] 1998 Oct; Vol. 11 (10), pp. 1214-21. - Publication Year :
- 1998
-
Abstract
- To evaluate the influence of the sodium/proton exchanger (Na+,H+ exchanger) on the constriction of rat resistance vessels and on the iliac artery, the isometric vasoconstrictions of renal resistance vessels and strips from iliac artery derived from Wistar-Kyoto rats were measured using a vessel myograph. The Na+,H+ exchanger was activated by intracellular acidification using propionic acid. Cytosolic pH (pHi) and cytosolic free sodium concentration ([Na+]i) in vascular smooth muscle cells were measured using the fluorescent dye technique. The activation of the Na+,H+ exchanger increased the [Na+]i by 12.4 +/- 1.3 mmol/L (n = 8). The activation of the Na+,H+ exchanger caused a contractile response of the renal resistance vessels (increase of tension, 1.5 +/- 0.1 x 10(-3) N; n = 13) and of the rat iliac artery (increase of tension, 7.5 +/- 0.8 x 10(-3) N; n = 5). The contractile response after activation of the Na+,H+ exchanger was significantly inhibited in the absence of external sodium or in the presence of amiloride, confirming the involvement of the Na+,H+ exchanger. The contractile response after activation of the Na+,H+ exchanger was significantly reduced in the absence of external calcium, after inhibition of calcium channels by nifedipine, and in the presence of an intracellular calcium antagonist 8-(diethylamino-)-octyl-3,4,5-trimethoxybenzoate (TMB-8), indicating that the activation of the Na+,H+ exchanger consecutively caused transplasma membrane calcium influx. On the other hand, the inhibition of the Na+,Ca2+ exchanger by NiCl2 significantly increased the vasoconstriction of renal resistance vessels after activation of the Na+,H+ exchanger. The activation of the Na+,H+ exchanger produces vasoconstriction by an increased cytosolic sodium concentration, inhibition of the Na+,Ca2+ exchanger, and activation of transplasma membrane calcium influx through potential dependent calcium channels.
- Subjects :
- Acids metabolism
Animals
Blood Vessels physiology
Cells, Cultured
Hydrogen-Ion Concentration
Iliac Artery physiology
Intracellular Membranes metabolism
Male
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular metabolism
Osmolar Concentration
Rats
Rats, Inbred WKY
Sodium metabolism
Renal Circulation physiology
Sodium-Hydrogen Exchangers physiology
Vascular Resistance physiology
Vasoconstriction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0895-7061
- Volume :
- 11
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- American journal of hypertension
- Publication Type :
- Academic Journal
- Accession number :
- 9799038
- Full Text :
- https://doi.org/10.1016/s0895-7061(98)00127-7