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Engineering of N-terminal threonines in the D1 protein impairs photosystem II energy transfer in Synechocystis 6803.
- Source :
-
FEBS letters [FEBS Lett] 1998 Oct 09; Vol. 436 (3), pp. 434-8. - Publication Year :
- 1998
-
Abstract
- Mutants of the cyanobacterium Synechocystis sp. PCC 6803 with N-terminal changes in the photosystem (PSII) II D1 protein were analysed by flash-induced oxygen evolution, chlorophyll a fluorescence decay kinetics and 77 K fluorescence emission spectra. The data presented here show that mutations of the Thr-2, Thr-3 and Thr-4 in D1 do not influence the oxygen evolution. A perturbation on the acceptor side was observed and the importance of the N-terminal threonines for an efficient energy transfer between the phycobilisome and PSII and for stability of the PSII complex was demonstrated.
- Subjects :
- Amino Acid Substitution
Chlorophyll metabolism
Chlorophyll A
Cyanobacteria genetics
Cyanobacteria growth & development
Darkness
Energy Transfer
Kinetics
Light
Light-Harvesting Protein Complexes
Oxygen metabolism
Photosystem II Protein Complex
Phycobilisomes
Protein Engineering
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Cyanobacteria metabolism
Photosynthetic Reaction Center Complex Proteins chemistry
Photosynthetic Reaction Center Complex Proteins metabolism
Threonine
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 436
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 9801164
- Full Text :
- https://doi.org/10.1016/s0014-5793(98)01179-x