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Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei.
- Source :
-
Molecular and biochemical parasitology [Mol Biochem Parasitol] 1998 Sep 15; Vol. 95 (2), pp. 229-40. - Publication Year :
- 1998
-
Abstract
- The complete sequence of the cdc2-related kinase 2 (CRK2) gene from Plasmodium knowlesi and from P. berghei was determined. In both species, the CRK2 gene is closely linked to an elongation factor 1 alpha gene. The two CRK2 proteins are highly homologous to the P. falciparum PfPK5 protein. The CRK2 gene of both species is expressed at a low level during the asexual cell-cycle within the host erythrocytes. The P. berghei CRK2 mRNA is also present in gametocytes and in stages during development in the mosquito, suggesting a role of this protein in different parts of the life cycle. A conserved sequence located in the 5' untranslated region immediately upstream of the initiator ATG has the potential to form a stem-loop structure. Although the CRK2 protein possesses most of the domains that are conserved among cdc2-proteins, neither a recombinant P. knowlesi CRK2 protein nor a recombinant P. berghei protein was able to complement a yeast cdc28ts mutant. Furthermore and in contrast to the P. falciparum PfPK5 protein, a recombinant monomeric P. knowlesi CRK2 protein showed no kinase activity.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
Conserved Sequence
DNA, Complementary
Gene Expression
Genes, Protozoan
Genetic Complementation Test
Molecular Sequence Data
Plasmodium berghei genetics
Plasmodium berghei growth & development
Plasmodium knowlesi genetics
Plasmodium knowlesi growth & development
Protozoan Proteins metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Protozoan genetics
RNA, Protozoan metabolism
Saccharomyces cerevisiae genetics
Sequence Analysis, DNA
Plasmodium berghei enzymology
Plasmodium knowlesi enzymology
Protein Kinases
Protozoan Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0166-6851
- Volume :
- 95
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular and biochemical parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 9803415
- Full Text :
- https://doi.org/10.1016/s0166-6851(98)00104-2