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Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake.
- Source :
-
International journal of systematic bacteriology [Int J Syst Bacteriol] 1998 Oct; Vol. 48 Pt 4, pp. 1405-12. - Publication Year :
- 1998
-
Abstract
- Four freshwater Antarctic lakes were examined for the presence of beta-galactosidase-producing bacteria using mineral medium enrichments and lactose. Enrichments from only one of the lakes produced growth and two strains were isolated that were very similar in phenotype and fatty acid profile, and shared considerable homology in their DNA (DNA-DNA hybridization = 93 +/- 7%). The strains were psychrotrophic with theoretical Tmax, Tmin and Topt of 30-31, -7 degrees and 26 degrees C, respectively. The beta-galactosidase in cell extracts had an optimal activity at 39 degrees C. The strains were Gram-negative rods, showed gliding motility, contained branched and hydroxy fatty acids, and menaquinone 6 as the major respiratory quinone. The strains did not form microcysts and utilized lactose while using ammonium ions as a source of nitrogen, and a range of other sugars. The G + C content of the DNA was 34 mol%. Phylogenetic analysis of one of the strains, by comparison of 16S rDNA sequences, showed that it was most similar, but not identical to, Flavobacterium columnare and '[Sporocytophaga] cauliformis'. Both species could be differentiated phenotypically from the Antarctic isolates. DNA-DNA hybridization of the Antarctic isolate with six different members of the Flavobacterium 16S rDNA cluster showed no strain with greater than 18% relatedness. The nearest type species to the Antarctic isolate in the phylogenetic analysis was Flavobacterium aquatile. The name Flavobacterium hibernum is proposed for the Antarctic strains, and the type strain is ATCC 51468T (= ACAM 376T).
- Subjects :
- Antarctic Regions
Base Composition
DNA, Bacterial chemistry
DNA, Ribosomal chemistry
Flavobacterium enzymology
Flavobacterium isolation & purification
Flavobacterium physiology
Lactose metabolism
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Temperature
Terminology as Topic
beta-Galactosidase metabolism
Flavobacterium classification
Fresh Water microbiology
Water Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0020-7713
- Volume :
- 48 Pt 4
- Database :
- MEDLINE
- Journal :
- International journal of systematic bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 9828443
- Full Text :
- https://doi.org/10.1099/00207713-48-4-1405