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Role of far upstream repressor elements controlling proto-Ha-ras gene transcription.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1998 Nov 27; Vol. 252 (3), pp. 716-22. - Publication Year :
- 1998
-
Abstract
- The far upstream region of the rat Ha-ras gene has been characterized to determine whether possible repressor sequences may control the low level of Ha-ras gene transcription from its TATA-less, GC-rich strong promoter. The chloramphenicol acetyl transferase (CAT) gene under the control of the 3.8-kb Ha-ras upstream promoter was minimally expressed in HeLa cells. Surprisingly, CAT gene expression was increased by the deletion of a 0.7-kb BglII fragment containing non-coding exon minus 2 and TATA box promoter elements located 1.7 kb upstream of the GC-rich strong promoter. Far upstream (CA)25 repeats also appeared to repress Ha-ras gene activity. Sequences within the 0.7-kb BglII fragment suppressed CAT gene expression when placed upstream of a heterologous thymidine kinase (tk) gene promoter. Repressor activity was further localized to a 160-bp AvrII-BglII sub-fragment. Gel shift assays identified two sequence-specific DNA binding proteins. The results demonstrated for the first time that far upstream repressor sequences control normal transcription of the Ha-ras proto-oncogene.<br /> (Copyright 1998 Academic Press.)
- Subjects :
- Animals
Chloramphenicol O-Acetyltransferase genetics
Deoxyribonuclease BamHI metabolism
Deoxyribonuclease HindIII metabolism
Deoxyribonucleases, Type II Site-Specific metabolism
Promoter Regions, Genetic
Rats
Sequence Analysis, DNA
Bacterial Proteins
Gene Expression Regulation
Genes, ras genetics
Repressor Proteins physiology
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 252
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 9837772
- Full Text :
- https://doi.org/10.1006/bbrc.1998.9711