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Differences in C/EBPs in normal tissue and papillomas of the larynx.
- Source :
-
Cell proliferation [Cell Prolif] 1998 Jun-Aug; Vol. 31 (3-4), pp. 127-38. - Publication Year :
- 1998
-
Abstract
- Transcription factors belonging to the family of CAAT enhancer binding proteins (C/EBPs) programme differentiation in a wide variety of cells. We asked about the expression of C/EBPs in squamous epithelium. Using immunohistochemistry, C/EBPalpha and C/EBPbeta were easily detected in tissue sections from normal larynx or laryngeal papillomas, benign tumours with a papillomavirus aetiology and characterized by abnormal differentiation. A temporal expression of these C/EBPs occured as keratinocytes differentiated. In both tissues, C/EBPbeta appeared to be exclusively nuclear. In normal tissue, the greatest amount of C/EBPbeta was present in the spinous layer, and much less occurred in the granular layer. The basal layer of the laryngeal papillomas contained the most C/EBPbeta. Less was present in the spinous layer. Little C/EBPbeta was in the granular layer. Much C/EBPalpha was in the cytoplasm in both tissues. In normal tissue, nuclear C/EBPalpha staining was virtually absent in the basal layer, and became present in the spinous layer. Nuclear C/EBPalpha was randomly distributed in all layers in papillomas. Using immunoblots and Southwestern blots, we detected abundant truncated isoforms of C/EBPalpha in the papillomas. Since differentiation of many tissues is determined by the relative amounts of different C/EBPs, our data supports a role for C/EBPs in the differentiation of squamous epithelium.
- Subjects :
- Blotting, Western
CCAAT-Enhancer-Binding Proteins
Cell Differentiation
Cell Nucleus chemistry
Dimerization
Epithelial Cells chemistry
Frozen Sections
HeLa Cells
Humans
Immunohistochemistry
Laryngeal Neoplasms pathology
Laryngeal Neoplasms virology
Molecular Weight
Papilloma pathology
Papilloma virology
Papillomaviridae
Response Elements genetics
DNA-Binding Proteins analysis
Laryngeal Neoplasms chemistry
Larynx chemistry
Nuclear Proteins analysis
Papilloma chemistry
Protein Isoforms analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7722
- Volume :
- 31
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Cell proliferation
- Publication Type :
- Academic Journal
- Accession number :
- 9853426