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A non-radioactive method for small RNA detection by northern blotting.
- Source :
- Rice (19398425); Dec2014, Vol. 7 Issue 1, p1-7, 7p
- Publication Year :
- 2014
-
Abstract
- Background: Small non-coding RNAs are essential regulators of gene expression at the transcriptional and posttranscriptional levels. High-throughput sequencing has revealed thousands of predicted small RNAs; however, only a few of these have been well characterized. Northern blotting is the most convincing method for small RNA validation. Findings: In this study, we improved the Northern blot method by using biotin-labeled probes. miRNAs and siRNAs derived from both Arabidopsis thaliana and Oryza sativa were investigated. The results suggest that this improved method is sensitive and efficient, with approximately 5 μg of total RNA being sufficient for detection. Furthermore, long-term storage of probes labeled in this manner is more convenient, less contaminative and degradative compared with traditional probes. Conclusions: This protocol is an alternative strategy for small RNA detection and represents an efficient means of researching small RNAs. [ABSTRACT FROM AUTHOR]
- Subjects :
- RADIOACTIVE contamination
RICE
NON-coding RNA
BLOTTING paper
PLANTING
Subjects
Details
- Language :
- English
- ISSN :
- 19398425
- Volume :
- 7
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Rice (19398425)
- Publication Type :
- Academic Journal
- Accession number :
- 100301557
- Full Text :
- https://doi.org/10.1186/s12284-014-0026-1