Back to Search Start Over

Promoter Region Hypermethylation and mRNA Expression of MGMT and p16 Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma.

Authors :
Bhatia, Vikram
Goel, Madhu Mati
Makker, Annu
Tewari, Shikha
Yadu, Alka
Shilpi, Priyanka
Kumar, Sandeep
Agarwal, S. P.
Goel, Sudhir K.
Source :
BioMed Research International; 2014, Vol. 2014, p1-10, 10p
Publication Year :
2014

Abstract

Promoter methylation and relative gene expression of O6-methyguanine-DNA-methyltransferase (MGMT) and p16 genes were examined in tissue and blood samples of patients with premalignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC). Methylation-specific PCR and reverse transcriptase PCR were performed in 146 tissue and blood samples from controls and patients with PMOLs and OSCC. In PMOL group, significant promoter methylation of MGMT and p16 genes was observed in 59% (P = 0.0010) and 57% (P = 0.0016) of tissue samples, respectively, and 39% (P = 0.0135) and 33% (P = 0.0074) of blood samples, respectively. Promoter methylation of both genes was more frequent in patients with OSCC, that is, 76% (P = 0.0001) and 82% (P = 0.0001) in tissue and 57% (P = 0.0002) and 70% (P = 0.0001) in blood, respectively. Significant downregulation of MGMT and p16 mRNA expression was observed in both tissue and blood samples from patients with PMOLs and OSCC. Hypermethylation-induced transcriptional silencing of MGMT and p16 genes in both precancer and cancer suggests important role of these changes in progression of premalignant state to malignancy. Results support use of blood as potential surrogate to tissue samples for screening or diagnosing PMOLs and early OSCC. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23146133
Volume :
2014
Database :
Complementary Index
Journal :
BioMed Research International
Publication Type :
Academic Journal
Accession number :
100578421
Full Text :
https://doi.org/10.1155/2014/248419