SSR markers related to pod characteristics in a new peanut cultivar Zhonghua 16 with high yield and oil content and its direct and indirect parents.

Pod traits including pod length and width, seed length and width, 100-pod weight, and 100-seed weight of a high yield and high oil content novel peanut cultivar Zhonghua 16 and 8 related parental lines in its breeding pedigree were investigated. The results indicated that the pod length, 100 - pod weight and 100 - seed weight of Zhonghua 16 were lower than the corresponding data of the immediate parental genotypes, Zhonghua 5 and 8130 respectively, but higher than the corresponding means of all 8 parental lines respectively. The pod width and seed width of Zhonghua 16 were higher than that of the 8 parental lines in the pedigree respectively. The seed length of Zhonghua 16 were higher than that of the Zhonghua 5 and 8130 and the average of the 8 parents respectively. Based on the results of PCR analysis using 136 polymorphic SSR primers, 64. 1% and 87. 3% polymorphic bands of 8130 and Zhonghua 5 were identified in Zhonghua 16. Besides, there were some bands in Zhonghua 16 which were absent in both direct parents 8130 and Zhonghua 5. Certain candidate SSR markers related to the pod traits were identified including 5 markers related to 100 - pod weight, 4 related to 100 - seed weight, 3 related to pod length and 3 related to seed length. Several SSR bands related to large pod including IPAHM606 - 1, IPAHM531-1, GA8 - 2, 1B9 - 4 and HAS0428 - 2 were observed to have been inherited from the parents to Zhonghua 16. [ABSTRACT FROM AUTHOR]