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Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA.
- Source :
- Scientific Reports; 2/6/2015, p8256, 1p
- Publication Year :
- 2015
-
Abstract
- Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in various somatic tissues and ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and reliable approach for generation of gene-modified pigs. [ABSTRACT FROM AUTHOR]
- Subjects :
- ZYGOTES
SWINE
MESSENGER RNA
TRANSFER-messenger RNA
RNA
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- Complementary Index
- Journal :
- Scientific Reports
- Publication Type :
- Academic Journal
- Accession number :
- 100878546
- Full Text :
- https://doi.org/10.1038/srep08256