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Depletion of regulatory T cells in a hapten-induced inflammation model results in prolonged and increased inflammation driven by T cells.
- Source :
- Clinical & Experimental Immunology; Mar2015, Vol. 179 Issue 3, p485-499, 15p, 1 Chart, 6 Graphs
- Publication Year :
- 2015
-
Abstract
- Regulatory T cells ( T<subscript>regs</subscript>) are known to play an immunosuppressive role in the response of contact hypersensitivity ( CHS), but neither the dynamics of T<subscript>regs</subscript> during the CHS response nor the exaggerated inflammatory response after depletion of T<subscript>regs</subscript> has been characterized in detail. In this study we show that the number of T<subscript>regs</subscript> in the challenged tissue peak at the same time as the ear-swelling reaches its maximum on day 1 after challenge, whereas the number of T<subscript>regs</subscript> in the draining lymph nodes peaks at day 2. As expected, depletion of T<subscript>regs</subscript> by injection of a monoclonal antibody to CD25 prior to sensitization led to a prolonged and sustained inflammatory response which was dependent upon CD8 T cells, and co-stimulatory blockade with cytotoxic T lymphocyte antigen-4-immunoglobulin ( CTLA-4- Ig) suppressed the exaggerated inflammation. In contrast, blockade of the interleukin ( IL)-10-receptor ( IL-10 R) did not further increase the exaggerated inflammatory response in the T<subscript>reg</subscript>-depleted mice. In the absence of T<subscript>regs</subscript>, the response changed from a mainly acute reaction with heavy infiltration of neutrophils to a sustained response with more chronic characteristics (fewer neutrophils and dominated by macrophages). Furthermore, depletion of T<subscript>regs</subscript> enhanced the release of cytokines and chemokines locally in the inflamed ear and augmented serum levels of the systemic inflammatory mediators serum amyloid ( SAP) and haptoglobin early in the response. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
HAPTENS
INFLAMMATION
IMMUNOSUPPRESSION
IMMUNOGLOBULINS
BLOOD serum analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00099104
- Volume :
- 179
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Clinical & Experimental Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 100989091
- Full Text :
- https://doi.org/10.1111/cei.12466