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Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats.
- Source :
- Chinese Journal of Integrative Medicine; Mar2015, Vol. 21 Issue 3, p188-195, 8p
- Publication Year :
- 2015
-
Abstract
- Objective: To investigate the effect of Dan-gua Fang (丹瓜方) on adenosine 5′-monophosphate (AMP) activated protein kinase (AMPK) α expression in liver and subsequent improvement of glucose and lipid metabolism. Methods: Forty 13-week-old diabetic Goto-Kakizaki (GK) rats were randomly divided into model, Dan-gua Fang, metformin and simvastatin groups (n=10 for each), and fed high-fat diet ad libitum. Ten Wistar rats were used as normal group and fed normal diet. After 24 weeks, liver expression of AMPKα mRNA was assessed by real-time PCR. AMPKα and phospho-AMPKα protein expression in liver was evaluated by Western blot. Liver histomorphology was carried out after hematoxylin-eosin staining, and blood glucose (BG), glycosylated hemoglobin A1c (HbA1c), food intake and body weight recorded. Results: Similar AMPKα mRNA levels were found in the Dan-gua Fang group and normal group, slightly higher than the values obtained for the remaining groups ( P<0.05). AMPKα protein expression in the Dan-gua Fang group animals was similar to other diabetic rats, whereas phospho-AMPKα (Thr-172) protein levels were markedly higher than in the metformin group and simvastatin group ( P<0.05), respectively. However, phosphor-AMPKα/AMPKα ratios were similar in all groups. Dan-gua Fang reduced fasting blood glucose with similar strength to metformin, and was superior in reducing cholesterol, triglycerides, high-density lipoprotein cholesterol as well as improving low-density lipoprotein cholesterol in comparison with simvastatin and metformin. Dan-gua Fang decreases plasma alanine aminotransferase (ALT) significantly. Conclusion: Dan-gua Fang, while treating phlegm-stasis, could decrease BG and lipid in type 2 diabetic GK rats fed with high-fat diet, and effectively protect liver histomorphology and function. This may be partly explained by increased AMPK expression in liver. Therefore, Dan-gua Fang might be an ideal drug for comprehensive intervention for glucose and lipid metabolism disorders in type 2 diabetes mellitus. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHINESE medicine
ANALYSIS of variance
ANIMAL experimentation
ANTHROPOMETRY
BLOOD sugar
COMBINATION drug therapy
CHOLESTEROL
HERBAL medicine
HISTOLOGICAL techniques
TYPE 2 diabetes
POLYMERASE chain reaction
RATS
RESEARCH funding
WESTERN immunoblotting
METABOLIC syndrome
DATA analysis software
DESCRIPTIVE statistics
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 21
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 101448051
- Full Text :
- https://doi.org/10.1007/s11655-014-1826-2