Detection of peanut viruses in Gaocheng of Hebei Province by RT-PCR and sequence analysis of its coat protein gene.

Peanut virus diseases can cause mosaic and dwarf symptoms on peanut plants and influence peanut's production. Reverse transcription PCR(RT-PCR)was used to detect peanut viruses in 12 peanut samples collected from Gaocheng, Hebei Province. Results revealed that only Peanut stripe virus (PStV) was detected in the 90% of the collected samples with mosaic and dwarf symptom. The coat protein gene (cp) of PStV of two isolates were sequenced, and their sizes were 864 base pairs. Sequence analysis showed that the sequences of Gaocheng isolates shared 94.4%-99.2% and 94.7%-99.7% similarities at nucleotide level and 95.5%-99.7% and 95.8%-100.0% at amino acid level with those of other PStV strains. These isolates shared closer relationship with Chinese and USA isolates compared with Chinese Taiwan, Thailand and Vietnam isolates by phylogenetic analysis of CP nucleotide sequence. [ABSTRACT FROM AUTHOR]