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Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics.
- Source :
- Nature Methods; Apr2015, Vol. 12 Issue 4, p319-322, 4p
- Publication Year :
- 2015
-
Abstract
- Focused-ion-beam scanning electron microscopy (FIB-SEM) has become an essential tool for studying neural tissue at resolutions below 10 nm × 10 nm × 10 nm, producing data sets optimized for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 μm thick) optimally sized and mounted for efficient, parallel FIB-SEM imaging. These chunks are imaged separately and then 'volume stitched' back together, producing a final three-dimensional data set suitable for connectome tracing. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 12
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 101861268
- Full Text :
- https://doi.org/10.1038/nmeth.3292