The Effect and Its Mechanism of Cisplatin on the Expression of PD-L1 in TC-1 Cell Surface.

Objective The study was aimed to investigate the effect and its mechanism of cisplatin on the expression of programmed death ligand-1 (PD-L1) in TC-1 cell surface, in order to provide an experimental basis to improve the therapeutic efficacy for cervical cancer. Methods The concentration of cisplatin was optimized by MTT method through detecting the cells proliferation vitality. Then, the expressions of PD-L1 in TC-1 cells treated by cisplatin and PKD blocker GÖ6976 were measured by immunohistochemistry. Results (1) The proliferation of TC-1 cells were inhibited by cisplatin with the IC₅₀ value of 25.5 µg/mL; (2) The expression of PD-L1 in TC-1 cell surface were (64.96 ± 11.55) % in cisplatin group, which was (39.59 ± 5.99) % in negative control group. There was a significant difference between the two groups (P < 0.05); (3) The expression of PD-L1 in TC-1 cell surface treated by cisplatin combined with PKD blocker GÖ6976 were (61.56 ± 3.43) % after 24 h, which were (79.54 ± 5.09) % in cisplatin group, and the difference was significant (P < 0.05). Conclusion Cisplatin can promote the expression of PD-L1 in TC-1 cell surface which could be significantly inhibited by the PKD pathway blocked. Hence, cisplatin might influence the expression of PD-L1 through PDK pathway. [ABSTRACT FROM AUTHOR]