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A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.
- Source :
- PLoS ONE; Jun2015, Vol. 10 Issue 6, p1-14, 14p
- Publication Year :
- 2015
-
Abstract
- A sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-distributed AuNPs, the enzyme-modified electrode shows an excellent electrochemical redox behavior. Compared with bare glass carbon electrode (GCE), the cathodic peak current of the enzymatic electrode increases 12.6 times at a formal potential of −100mV (vs. SCE) and charge-transfer resistance decreases 62.8%. Additionally, the AuNPs-CESM electrode exhibits a good biocompatibility, which effectively retains its bioactivity with a surface coverage of HRP 6.39×10<superscript>−9</superscript>mol cm<superscript>−2</superscript> (752 times higher than the theoretical monolayer coverage of HRP). Furthermore, the HRP-AuNPs-CESM-GCE electrode, as a biosensor for H<subscript>2</subscript>O<subscript>2</subscript> detection, has a good accuracy and high sensitivity with the linear range of 0.01–2.7 mM H<subscript>2</subscript>O<subscript>2</subscript>and the detection limit of 3μM H<subscript>2</subscript>O<subscript>2</subscript>(S/N = 3). [ABSTRACT FROM AUTHOR]
- Subjects :
- HYDROGEN peroxide
BIOSENSORS
GOLD nanoparticles
THREE-dimensional imaging
EGGSHELLS
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 103567151
- Full Text :
- https://doi.org/10.1371/journal.pone.0130156