Back to Search
Start Over
Effects of additives on the lyophilized and thermal stability of d-galactose-6-sulfurylase activity from Eucheuma striatum (Rhodophyta).
- Source :
- Journal of Applied Phycology; Aug2015, Vol. 27 Issue 4, p1709-1715, 7p
- Publication Year :
- 2015
-
Abstract
- d-galactose-6-sulfurylase has potential application in the improvement of the carrageenan quality. The lyophilized and thermal stability of the Gal-6-sulfurylase prepared from Eucheuma striatum was investigated using selected chemical additives. The addition of 5 % ( w/ v) sucrose and trehalose to Gal-6-sulfurylase prior to lyophilization could retain nearly 100 % enzymatic activity, compared to only 19 % for the lyophilized control. The lyophilized Gal-6-sulfurylase containing sucrose maintained full activity for even 40 days storage at −20 °C. For Gal-6-sulfurylase solution, glycerol was effective for long-term stability at −20 °C. Moreover, polyol sucrose and amino acid glycine enhanced the thermal stability of Gal-6-sulfurylase, while polyol mannitol decreased the thermal stability of Gal-6-sulfurylase. The results show that the Gal-6-sulfurylase, found in the red alga E. striatum, was stabilized by the addition of chemical additives. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09218971
- Volume :
- 27
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Journal of Applied Phycology
- Publication Type :
- Academic Journal
- Accession number :
- 103670229
- Full Text :
- https://doi.org/10.1007/s10811-014-0466-4