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The stability of free-[beta] human chorionic gonadotrophin and pregnancy-associated plasma protein-A in first trimester dried blood spots.
- Source :
- Prenatal Diagnosis; Mar2011, Vol. 31 Issue 3, p293-298, 6p
- Publication Year :
- 2011
-
Abstract
- OBJECTIVES: To determine the stability of first trimester free-[beta] human chorionic gonadotrophin (free-[beta] hCG) and pregnancy-associated plasma protein-A (PAPP-A) in dried blood spots (DBSs) under typical storage conditions. METHODS: First trimester maternal blood was spotted onto filter paper and left to dry. DBSs were analysed for PAPP-A and free-[beta] hCG using an AutoDELFIA dual assay at t = 0. Cards were stored at one of - 20 °C, refrigerator temperature, room temperature or 30 °C and reanalysed at set future time points. RESULTS: Free-[beta] hCG was stable (<10% change in concentration) under all temperatures tested for at least 35 days. PAPP-A was stable at - 20 °C and refrigerator temperature for at least 35 days. However, PAPP-A levels decreased by 10% at 4.1 days at room temperature and at 3.9 days at 30 °C. Longer-term storage at - 20 °C and refrigerator temperature showed that both PAPP-A and free-[beta] hCG levels were significantly decreased by 107 and 244 days. CONCLUSIONS: Free-[beta] hCG stability is greatly improved in DBS compared to serum storage; however PAPP-A stability is decreased in the DBS medium. Despite this DBS, screening may not necessitate such strict storage and transportation rules compared to serum screening programmes. Copyright © 2011 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01973851
- Volume :
- 31
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Prenatal Diagnosis
- Publication Type :
- Academic Journal
- Accession number :
- 104836226
- Full Text :
- https://doi.org/10.1002/pd.2709