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Recruitment of IL-27-Producing CD4 T Cells and Effect of IL-27 on Pleural Mesothelial Cells in Tuberculous Pleurisy.
- Source :
- Lung; Aug2015, Vol. 193 Issue 4, p539-548, 10p
- Publication Year :
- 2015
-
Abstract
- Background: The numbers of IL-27-producing CD4 T cells and the concentration of soluble IL-27 have been found to be increased in tuberculous pleural effusion (TPE). The objective of the present study was to explore the mechanism by which IL-27CD4 T cells are recruited into the pleural space, and to explore the impact of IL-27 on pleural mesothelial cells (PMCs). Methods: The expression profiles of chemokine receptor (CCR) were determined by flow cytometry. The chemoattractant activity of chemokines CCL20 and CCL22 for IL-27CD4 T cells in vitro was observed. Effects of IL-27 on wound healing, proliferation and apoptosis of PMCs were also investigated. Results: IL-27CD4 T cells in TPE expressed high level of CCR6, medium level of CCR4, and low levels of CCR2, CCR3, CCR5, CCR7, CCR10, and CXCR3. Recruitment of IL-27CD4 T cells into TPE could be induced by pleural CCL20 and CCL22. By activating STAT3 signaling, IL-27 significantly improved wound healing and promoted proliferation of PMCs, and completely prevented apoptosis of PMCs induced by IFN-γ. Conclusions: After being recruited into pleural space by CCL20 or/and CCL22, these pleural IL-27-producing CD4 T cells may play important roles in tuberculosis immunity by affecting PMC functions. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
PLEURAL effusions
CHEMOKINE receptors
FLOW cytometry
CHEMOKINES
Subjects
Details
- Language :
- English
- ISSN :
- 03412040
- Volume :
- 193
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Lung
- Publication Type :
- Academic Journal
- Accession number :
- 108303486
- Full Text :
- https://doi.org/10.1007/s00408-015-9738-2