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Paradoxical effects of interleukin-10 on the maturation of murine myeloid dendritic cells.
- Source :
- Immunology; Oct2003, Vol. 110 Issue 2, p188-196, 8p
- Publication Year :
- 2003
-
Abstract
- Summary The immunoregulatory cytokine, interleukin-10 (IL-10), has been shown to inhibit the maturation of human myeloid dendritic cells (DC). In the present study, we demonstrate that IL-10 has paradoxical effects on the maturation of murine myeloid bone marrow-derived DC. On the one hand, IL-10 inhibits the maturation of murine myeloid DC. The addition of IL-10 to granulocyte–macrophage colony-stimulating factor (GM-CSF)-supported murine BM-derived DC cultures reduced the frequency of major histocompatibility complex (MHC) class II[sup bright] cells. These IL-10-pretreated DC have a reduced capacity to stimulate T cells in an allogeneic mixed leucocyte reaction. On the other hand, however, and in contrast to the effects of IL-10 on human DC, we found that the addition of IL-10 from the initiation of the culture onwards induced an up-regulation of the expression of the costimulatory molecules CD40, CD80 and CD86 on murine myeloid DC, as compared to DC generated with GM-CSF only. Moreover, a subpopulation of IL-10-pretreated MHC class II[sup dim] DC lacked the capacity to take up dextran-fluorescein isothiocyanate (FITC), a feature of DC maturation. Taken together, our data demonstrate that the generation of murine myeloid DC in the presence of IL-10 results in a population of incompletely matured MHC class II[sup dim] CD80[sup +] CD86[sup +] DC. These DC lack T-cell stimulatory capacity, suggesting a role for IL-10 in conferring tolerogenic properties on murine myeloid DC. [ABSTRACT FROM AUTHOR]
- Subjects :
- INTERLEUKINS
DENDRITIC cells
BONE marrow
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 110
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 10848867
- Full Text :
- https://doi.org/10.1046/j.1365-2567.2003.01730.x