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Fibroblasts support migration of monocyte-derived dendritic cells by secretion of PGE2 and MMP-1.
- Source :
- Experimental Dermatology; Aug2015, Vol. 24 Issue 8, p598-604, 7p
- Publication Year :
- 2015
-
Abstract
- The outcome of a cutaneous immune response is critically dependent upon the ability of dendritic cells ( DC) to migrate from skin to the draining lymph nodes - a process that is influenced by the cutaneous tissue microenvironment. Here, the role of fibroblasts - a major component of the dermal microenvironment - on the migratory capacity of monocyte-derived DC (Mo DC) was investigated in a 3D collagen I matrix. Indeed, dermal fibroblasts supported the migration of pre-activated Mo DC through a 3D collagen I matrix. Activation of human Mo DC resulted in the release of TNFα and IL-1β that in turn stimulated MMP-1 (human collagenase) and PGE<subscript>2</subscript> secretion by human dermal fibroblasts. Transmigration assays confirmed the importance of fibroblast-derived MMP-1 and PGE<subscript>2</subscript> for the migration of Mo DC through a 3D collagen I matrix. Finally, in mice initiation of inflammation by induction of an irritant contact dermatitis or a psoriasis-like skin inflammation, the expression of the PGE<subscript>2</subscript> generating cox-2 and the mouse collagen I degrading enzyme matrix metalloproteinases ( MMP)-13 was strongly up-regulated. Our study indicates that Mo DC are able to instruct dermal fibroblasts resulting in enhanced migratory capability of Mo DC, thus highlighting the role of a crosstalk of DC with their stromal microenvironment for the control of cutaneous immune responses. [ABSTRACT FROM AUTHOR]
- Subjects :
- DENDRITIC cells
FIBROBLASTS
IMMUNE response
COLLAGENASES
SKIN inflammation
Subjects
Details
- Language :
- English
- ISSN :
- 09066705
- Volume :
- 24
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Experimental Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 108593277
- Full Text :
- https://doi.org/10.1111/exd.12722