Back to Search
Start Over
Enhanced differentiation of intraepithelial lymphocytes in the intestine of polymeric immunoglobulin receptor-deficient mice.
- Source :
- Immunology; Sep2015, Vol. 146 Issue 1, p59-69, 11p
- Publication Year :
- 2015
-
Abstract
- To clarify the effect of secretory IgA (s IgA) deficiency on gut homeostasis, we examined intraepithelial lymphocytes ( IELs) in the small intestine ( SI) of polymeric immunoglobulin receptor-deficient ( pIgR<superscript>−/−</superscript>) mice. The p IgR<superscript>−/−</superscript> mice exhibited the accumulation of CD8 αβ<superscript>+</superscript> T-cell receptor ( TCR)- αβ<superscript>+</superscript> IELs ( CD8 αβ<superscript>+</superscript> αβ- IELs) after weaning, but no increase of CD8 αβ<superscript>+</superscript> γδ- IELs was detected in pIgR<superscript>−/−</superscript> TCR- β<superscript>−/−</superscript> mice compared with pIgR<superscript>+/+</superscript> TCR- β<superscript>−/−</superscript> mice. When 5-bromo-2′-deoxyuridine (BrdU) was given for 14 days, the proportion of BrdU-labelled cells in SI- IELs was not different between pIgR<superscript>+/+</superscript> mice and pIgR<superscript>−/−</superscript> mice. However, the proportion of BrdU-labelled CD8 αβ<superscript>+</superscript>- IELs became higher in pIgR<superscript>−/−</superscript> mice than pIgR<superscript>+/+</superscript> mice 10 days after discontinuing BrdU-labelling. Intravenously transferred splenic T cells migrated into the intraepithelial compartments of pIgR<superscript>+/+</superscript> TCR- β<superscript>−/−</superscript> mice and pIgR<superscript>−/−</superscript> TCR- β<superscript>−/−</superscript> mice to a similar extent. In contrast, in the case of injection of immature bone marrow cells, CD8 αβ<superscript>+</superscript> αβ- IELs increased much more in the SI of pIgR<superscript>−/−</superscript> TCR- β<superscript>−/−</superscript> mice than pIgR<superscript>+/+</superscript> TCR- β<superscript>−/−</superscript> mice 8 weeks after the transfer. αβ- IELs from pIgR<superscript>−/−</superscript> mice could produce more interferon- γ and interleukin-17 than those of pIgR<superscript>+/+</superscript> mice, and intestinal permeability tended to increase in the SI of pIgR<superscript>−/−</superscript> mice with aging. Taken together, these results indicate that activated CD8 αβ<superscript>+</superscript> αβ- IELs preferentially accumulate in pIgR<superscript>−/−</superscript> mice through the enhanced differentiation of immature haematopoietic precursor cells, which may subsequently result in the disruption of epithelial integrity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 146
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 108696419
- Full Text :
- https://doi.org/10.1111/imm.12480