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Improving performance of Yarrowia lipolytica lipase lip2-catalyzed kinetic resolution of ( R, S )-1-phenylethanol by solvent engineering.
- Source :
- Biocatalysis & Biotransformation; Feb2015, Vol. 33 Issue 1, p38-43, 6p, 1 Black and White Photograph, 2 Charts, 3 Graphs
- Publication Year :
- 2015
-
Abstract
- ExtracellularYarrowia lipolyticalipase Lip2 (YLIP2) demonstrated an (R)-enantiopreference for efficient resolution of (R,S)-1-phenylethanol by solvent engineering with different kinds of binary solvent. The enantioselectivity was significantly improved by the addition of 1, 4-dioxane. The reaction parameters including co-solvent concentration, reaction temperature, and the reaction time were optimized. When the reaction was carried out with n-hexane in the presence of 0.8% 1,4-dioxane at 50°C for 72 h, the enantiomeric excess of product markedly increased to 99.1% from 66% in pure n-hexane; the enantiomeric ratio was higher than 200, which was 500-fold compared with that in pure n-hexane. The results indicated that it is very important to design the proper co-solvents, especially to create appropriate micro-environment for YLIP2 for catalyzing the resolution of (R,S)-1-phenylethanol. [ABSTRACT FROM AUTHOR]
- Subjects :
- KINETIC resolution
LIPASES
DIOXANE
ENANTIOMERS
HEXANE
Subjects
Details
- Language :
- English
- ISSN :
- 10242422
- Volume :
- 33
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Biocatalysis & Biotransformation
- Publication Type :
- Academic Journal
- Accession number :
- 108697525
- Full Text :
- https://doi.org/10.3109/10242422.2015.1018190