Abietane Diterpenoids from Perovskia atriplicifolia and Their Anti-HBV Activities.

Bioassay-guided phytochemical investigation on the 90% EtOH extract of Perovskia atriplicifolia resulted in the isolation of eight abietane diterpenoids, including three new ones (1-3). Based on spectroscopic methods involving 1D and 2D NMR spectroscopy techniques, mass spectrometry, and optical rotation, the structures of the new compounds (1-3) were unambiguously characterized. Compounds 1-2 and 4-8 were evaluated for their anti-HBV (hepatitis B virus) activity in HepG 2.2.15 cell line. Results suggested rosmadial (8) had the most anti-HBV potency, suppressing the secretion of HBsAg and HBeAg, with IC₅₀ values of 0.09 and 0.34 mM, respectively. [ABSTRACT FROM AUTHOR]