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Photodynamic Activities of Sulfonamide Derivatives of Porphycene on Nasopharyngeal Carcinoma Cells.

Authors :
Nai-Ki Mak
Tsz-Wai Kok
Ngok-Shun Wong, Ricky
Sum-Wai Lam
Yan-Kin Lau
Wing-Nang Leung
Nai-Ho Cheung
Huang, Dolly P.
Lam-Lung Yeung
Chang, Chi K.
Source :
Journal of Biomedical Science; Jul/Aug2003, Vol. 10 Issue 4, p418-429, 12p
Publication Year :
2003

Abstract

Two sulfonamide derivatives of porphycene, namely PS6 and PS6A, were synthesized, and their photodynamic efficacies on the nasopharyngeal carcinoma (NPC) cell line NPC/CNE-2 were evaluated. By comparing the 50% lethal concentrations (LC[sub50]) of these photosensitizers, we found that PS6A with a cationic ammonium group on the side chain exhibited potent photocytotoxicity on the NPC cell line. At a light dose of 1 J/cm² the LC[sub50] values of PS6 and PS6A for NPC cells were 11.6 and 1.92 µM, respectively. CNE-2 was found to rapidly take up PS6A in the first hour of incubation, and the uptake kinetics steadily increased to a plateau level after 18 h of incubation. The uptake of PS6A was temperature dependent. Over 99% of CNE-2 cells were sensitized by PS6A 24 h after drug treatment. Collapse of the mitochondrial membrane potential was also observed in PS6A photodynamic therapy (PDT)-treated CNE-2 cells 1.5 h after PDT. Confocal microscopy revealed that PS6A was predominantly localized in the mitochondria, lysosomes and Golgi bodies of NPC cells. Significant genotoxicity was not observed in CNE-2 cells. In functional studies, the in vitro formation of a capillary-like network of human umbilical vein endothelial cells in Matrigel was greatly inhibited by PS6A PDT in a dose-dependent manner. In conclusion, PS6A mediates both in vitro antitumor and antiangiogenic activities. PS6A might be a candidate for photodynamic treatment of NPCs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10217770
Volume :
10
Issue :
4
Database :
Complementary Index
Journal :
Journal of Biomedical Science
Publication Type :
Academic Journal
Accession number :
10891037
Full Text :
https://doi.org/10.1007/BF02256433