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Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB.
- Source :
- BioMed Research International; 3/30/2015, Vol. 2015, p1-6, 6p
- Publication Year :
- 2015
-
Abstract
- Retinoic acid-inducible gene I- (RIG-I-) like receptors (RLRs) have recently been identified as cytoplasmic sensors for viral RNA. RIG-I, a member of RLRs family, plays an important role in innate immunity. Although previous investigations have proved that RIG-I is absent in chickens, it remains largely unknown whether the chicken can respond to RIG-I ligand. In this study, the eukaryotic expression vectors encoding duRIG-I full length (duck RIG-I, containing all domains), duRIG-I N-terminal (containing the two caspase activation and recruitment domain, CARDs), and duRIG-I C-terminal (containing helicase and regulatory domains) labeled with 6*His tags were constructed successfully and detected by western blotting. Luciferase reporter assay and enzyme-linked immunosorbent assay (ELISA) detected the duRIG-I significantly activated NF-κB and induced the expression of IFN-β when polyinosinic-polycytidylic acid (poly[I:C], synthetic double-stranded RNA) challenges chicken embryonic fibroblasts cells (DF1 cells), while the duRIG-I was inactive in the absence of poly[I:C]. Further analysis revealed that the CARDs (duRIG-I-N) induced IFN-β production regardless of the presence of poly[I:C], while the CARD-lacking duRIG-I (duRIG-I-C) was not capable of activating downstream signals. These results indicate that duRIG-I CARD domain plays an important role in the induction of IFN-β and provide a basis for further studying the function of RIG-I in avian innate immunity. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANALYSIS of variance
ANIMAL experimentation
BIOLOGICAL assay
CELL culture
ENZYME-linked immunosorbent assay
FIBROBLASTS
GENES
GENETIC techniques
IMMUNITY
INTERFERONS
NUCLEOTIDES
POULTRY
RESEARCH funding
T-test (Statistics)
TRETINOIN
WESTERN immunoblotting
DNA-binding proteins
DATA analysis software
DESCRIPTIVE statistics
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2015
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 109273844
- Full Text :
- https://doi.org/10.1155/2015/348792