Anti-hyperalgesic activity of crude extract and 7-methyljuglone of Diospyros lotus roots.

This study was designed to evaluate the antihyperalgesic effect of crude extract ofDiospyros lotusfollowed by the isolation and characterisation of 7-methyljuglone in acetic acid and formalin tests. The pretreatment of crude extract evoked dose-dependent inhibition of noxious stimulation with maximum effect of 56.78% in acetic acid-induced writhing test, which were 51.89% and 60.69% in first and second phases, respectively, at 100 mg/kg i.p. The structure of 7-methyljuglone was confirmed by spectroscopic analysis. 7-Methyljuglone evoked profound increase in pain threshhold dose dependently; when it was studied in acetic acid-induced writhing test with 63.73% pain attenuation while 51.22% and 65.44% pain amelioration in first and second phases, respectively, at 100 mg/kg i.p. In conclusion, crude extract and 7-methyljuglone ofD. lotusroots possessed both peripheral and central antinociceptive potential and thus could be a useful new therapeutic agent. [ABSTRACT FROM PUBLISHER]