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Pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation: the Klebsiella paradigm.
- Source :
- Biology Direct; 9/30/2015, Vol. 10 Issue 1, p1-12, 12p, 5 Diagrams, 1 Chart, 2 Graphs
- Publication Year :
- 2015
-
Abstract
- Background: Various methods are currently used to define species and are based on the phylogenetic marker 16S ribosomal RNA gene sequence, DNA-DNA hybridization and DNA GC content. However, these are restricted genetic tools and showed significant limitations. Results: In this work, we describe an alternative method to build taxonomy by analyzing the pan-genome composition of different species of the Klebsiella genus. Klebsiella species are Gram-negative bacilli belonging to the large Enterobacteriaceae family. Interestingly, when comparing the core/pan-genome ratio; we found a clear discontinuous variation that can define a new species. Conclusions: Using this pan-genomic approach, we showed that Klebsiella pneumoniae subsp. ozaenae and Klebsiella pneumoniae subsp. rhinoscleromatis are species of the Klebsiella genus, rather than subspecies of Klebsiella pneumoniae. This pan-genomic analysis, helped to develop a new tool for defining species introducing a quantic perspective for taxonomy. Reviewers: This article was reviewed by William Martin, Pierre Pontarotti and Pere Puigbo (nominated by Dr Yuri Wolf). [ABSTRACT FROM AUTHOR]
- Subjects :
- CLADISTIC analysis
NUCLEIC acid hybridization
KLEBSIELLA pneumoniae
TAXONOMY
Subjects
Details
- Language :
- English
- ISSN :
- 17456150
- Volume :
- 10
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Biology Direct
- Publication Type :
- Academic Journal
- Accession number :
- 110084301
- Full Text :
- https://doi.org/10.1186/s13062-015-0085-2