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Major Histocompatibility Complex Class II Dextramers: New Tools for the Detection of antigen-Specific, CD4 T Cells in Basic and Clinical Research.
- Source :
- Scandinavian Journal of Immunology; Nov2015, Vol. 82 Issue 5, p399-408, 10p
- Publication Year :
- 2015
-
Abstract
- The advent of major histocompatibility complex ( MHC) tetramer technology has been a major contribution to T cell immunology, because tetramer reagents permit detection of antigen-specific T cells at the single-cell level in heterogeneous populations by flow cytometry. However, unlike MHC class I tetramers, the utility of MHC class II tetramers has been less frequently reported. MHC class II tetramers can be used successfully to enumerate the frequencies of antigen-specific CD4 T cells in cells activated in vitro, but their use for ex vivo analyses continues to be a problem, due in part to their activation dependency for binding with T cells. To circumvent this problem, we recently reported the creation of a new generation of reagents called MHC class II dextramers, which were found to be superior to their counterparts. In this review, we discuss the utility of class II dextramers vis-a-vis tetramers, with respect to their specificity and sensitivity, including potential applications and limitations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03009475
- Volume :
- 82
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Scandinavian Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 110220901
- Full Text :
- https://doi.org/10.1111/sji.12344