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Lipoteichoic acid upregulates NF-κB and proinflammatory cytokines by modulating β-catenin in bronchial epithelial cells.
- Source :
- Molecular Medicine Reports; 2015, Vol. 12 Issue 3, p4720-4726, 7p
- Publication Year :
- 2015
-
Abstract
- Lipoteichoic acid (LTA) is a major cell wall component and virulence factor of gram-positive bacteria. The present study investigated the LTA-induced inflammatory response of BEAS-2B human bronchial epithelial cells, and detected the expression levels of proinflammatory cytokines interleukin (IL)-6, IL-8, IL-1β, tumour necrosis factor-a and monocyte chemotactic protein-1, the upregulation of NF-κB, and the phosphorylation and degradation of I-κB. During the LTA-induced inflammatory response of the BEAS-2B human bronchial epithelial cells, the activity levels of the β-catenin-dependent promoter, and the protein expression levels of β-catenin were significantly upregulated, whereas β-catenin phosphorylation and the expression levels of AXIN were significantly downregulated. Following knockdown of β-catenin by small interfering (si)RNA transfection, both the LTA-induced protein expression levels of NF-κB and the LTA-induced activity levels of the NF-κB-dependent promoter were significantly reduced. Similarly, a marked reduction in I-κB phosphorylation and degradation was observed following β-catenin knockdown. The expression levels of the LTA-induced proinflammatory cytokines were also significantly reduced following β-catenin siRNA. These results suggest that β-catenin has a significant role in the regulation of NF-κB activity and proinflammatory cytokine expression during the LTA-induced inflammatory response of bronchial epithelial cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- LIPOTEICHOIC acid
CYTOKINES
CATENINS
EPITHELIAL cells
BRONCHI physiology
Subjects
Details
- Language :
- English
- ISSN :
- 17912997
- Volume :
- 12
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Molecular Medicine Reports
- Publication Type :
- Academic Journal
- Accession number :
- 110381265
- Full Text :
- https://doi.org/10.3892/mmr.2015.3965