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Establishment and characterization of a new marine fish cell line from ovary of barfin flounder ( Verasper moseri).
- Source :
- Journal of Ocean University of China; Dec2015, Vol. 14 Issue 6, p1105-1110, 6p
- Publication Year :
- 2015
-
Abstract
- A novel continuous ovary cell line from barfin flounder ( Verasper moseri) (BFO cell line) was established with its primitive application in transgenic expression demonstrated in this study. Primarily cultured cells grew well at 22°C in Dulbecco's modified Eagle medium/F12 medium (DMEM/F12, 1:1; pH 7.2) supplemented with 20% fetal bovine serum (FBS), carboxymethyl chitooligosaccharide, basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I). The primary BFO cells in fibroblastic morphology proliferated into a confluent monolayer about 2 weeks later, and were able to be subcultured. Impacts of medium and temperature on the growth of the cells were examined. The optimum growth was found in DMEM/F12 with 20% FBS and at 22°C. The BFO cells can be continuously subcultured to Passage 120 steadily with a population doubling time of 32.7 h at Passage 60. Chromosome analysis revealed that 72% of BFO cells at Passage 60 maintained the normal diploid chromosome number (46) with a normal karyotype of 2st+44t. The results of gene transformation indicated that green fluorescence protein (GFP) positively expressed in these cells after being transformed with pcDNA3.1-GFP. Therefore, a continuous and transformable BFO cell line was successfully established, which may serve as a useful tool for cytotechnological manipulation and transgenic modification of this fish. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16725182
- Volume :
- 14
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Journal of Ocean University of China
- Publication Type :
- Academic Journal
- Accession number :
- 110811500
- Full Text :
- https://doi.org/10.1007/s11802-015-2920-6