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Role of Phospholipase C-L2, a Novel Phospholipase C-Like Protein That Lacks Lipase Activity, in B-Cell Receptor Signaling.
- Source :
- Molecular & Cellular Biology; Oct2003, Vol. 23 Issue 20, p7329-7338, 10p, 38 Graphs
- Publication Year :
- 2003
-
Abstract
- Phospholipase C (PLC) plays important roles in phosphoinositide turnover by regulating the calciumprotein kinase C signaling pathway. PLC-L2 is a novel PLC-like protein which lacks PLC activity, although it is very homologous with PLC δ. PLC-L2 is expressed in hematopoietic cells, but its physiological roles and intracellular functions in the immune system have not yet been clarified. To elucidate the physiological function of PLC-L2, we generated mice which had a genetic PLC-L2 deficiency. PLC-L2-deficient mice grew with no apparent abnormalities. However, mature B cells from PLC-L2-deficient mice were hyperproliferative in response to B-cell receptor (BCR) cross-linking, although B2 cell development appeared to be normal. Molecular biological analysis revealed that calcium influx and NFATc accumulation in nuclei were increased in PLC-L2-deficient B cells. Extracellular signal-regulated kinase activity was also enhanced in PLC-L2deficient B cells. These mice had a stronger T-cell-independent antigen response. These results indicate that PLC-L2 is a novel negative regulator of BCR signaling and immune responses. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHOSPHOLIPASE C
PROTEIN kinase C
CELLULAR signal transduction
Subjects
Details
- Language :
- English
- ISSN :
- 02707306
- Volume :
- 23
- Issue :
- 20
- Database :
- Complementary Index
- Journal :
- Molecular & Cellular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 11146376
- Full Text :
- https://doi.org/10.1128/MCB.23.20.7329-7338.2003