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A rigid lanthanide binding tag to aid NMR studies of a 70 kDa homodimeric coat protein of human norovirus.
- Source :
- Chemical Communications; 1/11/2016, Vol. 52 Issue 3, p601-604, 4p
- Publication Year :
- 2016
-
Abstract
- Attachment of human noroviruses to histo blood group antigens is thought to be essential for infection of host cells. Molecular details of the attachment process can be studied in vitro using a variety of NMR experiments. The use of protein NMR based experiments requires assignments of backbone NMR signals. Using uniformly <superscript>2</superscript>H,<superscript>15</superscript>N-labeled protruding domains (P-dimers) of a prevalent epidemic human norovirus strain (GII.4 Saga) we have studied the potential of α-l-fucose covalently linked to a rigid lanthanide binding tag to aid backbone assignments using the paramagnetic properties of lanthanide ions. The synthesis of tagged α-l-fucose is reported. Notably, the metal chelating unit connects to the carbohydrate via a triazole linker constructed using click chemistry. [ABSTRACT FROM AUTHOR]
- Subjects :
- NOROVIRUSES
RARE earth metals
CALICIVIRUSES
RNA viruses
CLASS A metals
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 52
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 111937539
- Full Text :
- https://doi.org/10.1039/c5cc05827a